Pattern discontinuity, polarity and directional intercalation in axolotl limbs
نویسندگان
چکیده
منابع مشابه
Pattern discontinuity, polarity and directional intercalation in axolotl limbs.
Axolotl limb stumps with dorsal-ventral confrontations between digits 2 and 3 but with a normal anterior-posterior pattern were created by grafting between contralateral limbs. Graft and host differed in ploidy to permit a determination of the origin of cells in the regenerated limb. After regeneration, limbs were analysed for skeletal and muscle patterns and for the distribution of marked cell...
متن کاملRetinoic acid coordinately proximalizes regenerate pattern and blastema differential affinity in axolotl limbs.
An assay that detects position-related differences in affinity of axolotl regeneration blastema cells in vivo was used to test whether retinoic acid, which proximalizes regenerate pattern, simultaneously proximalizes blastema cell affinity. The assay involved autografting or homografting late bud forelimb blastomas derived from the wrist, elbow or midupper arm levels to the dorsal surface of th...
متن کاملPitx1 expression in developing and regenerating axolotl limbs.
The expression of the homeobox transcription factor Pitx1 was investigated in the Mexican axolotl (Ambystoma mexicanum) during limb development and regeneration by whole-mount mRNA in situ hybridizations. This clone shares high amino acid identity with Pitx1 from other vertebrates (92% Xenopus; 87% chick; 75% human and mouse) within the region isolated. In the developing limbs, Pitx1 was expres...
متن کاملRegulation of HoxA expression in developing and regenerating axolotl limbs.
Homeobox genes are important in the regulation of outgrowth and pattern formation during limb development. It is likely that homeobox genes play an equally important role during limb regeneration. We have isolated and identified 17 different homeobox-containing genes expressed by cells of regenerating axolotl limbs. Of these, nearly half of the clones represent genes belonging to the HoxA compl...
متن کاملIntercalation and the cellular origin of supernumerary limbs in Xenopus.
The hypothesis that a specialized polarizing zone controls the pattern of the anterior-posterior axis during limb development in Xenopus has been tested by analysing the cellular contribution to supernumerary limbs. Supernumerary limbs were generated by grafting hindlimb buds contralaterally between X. borealis and X. laevis to appose anterior and posterior limb tissues. Cells derived from thes...
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ژورنال
عنوان ژورنال: Development
سال: 1986
ISSN: 1477-9129,0950-1991
DOI: 10.1242/dev.93.1.51